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frozen human bone marrow mononuclear cells  (Lonza)


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    Lonza frozen human bone marrow mononuclear cells
    Frozen Human Bone Marrow Mononuclear Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+bone+marrow+mononuclear+cells/human+bone+marrow+mononuclear+cells/pmc12036193-31-0-10
    Average 90 stars, based on 1 article reviews
    frozen human bone marrow mononuclear cells - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Reversal of established liver fibrosis by IC-2-engineered mesenchymal stem cell sheets
    Article Snippet: For experiments using bone marrow mononuclear cells adherent to culture dishes and CD90 + /CD271 + cells, human bone marrow mononuclear cells were purchased from Lonza Inc. (Walkersville, MD) and were used at passage five as previously described .

    Article Title: The effects of biomimetically conjugated VEGF on osteogenesis and angiogenesis of MSCs (human and rat) and HUVECs co-culture models.
    Article Snippet: Proliferation study of HUVECs and MSCs co-culture Human MSCs were isolated from human bone marrow mononulear cells (Lonza, UK) by culturing in flasks coated with fibronectin R&D System, UK) in low glucose DMEM (Biosera, UK) supple- ented with 10% FBS and 1% A + A under 37 ◦C and 5% CO2.

    Cell Culture:

    Article Title: IL-12/IL-23p40 identified as a downstream target of apremilast in ex vivo models of arthritis
    Article Snippet: Culture supernatants were analyzed for the concentration of IL-10 and MCP-1 by enzyme-linked immunosorbent assays (ELISAs; Biolegend, San Diego, California, USA) and for enzyme activity of tartrate-resistant acid phosphatase (TRAP) positive by an enzymatic assay (B-bridge International, Santa Clara, California, USA) following the instructions of the manufacturer. .. Human bone marrow mononuclear cells (Lonza, Walkersville, MA, USA) were cultured in 10% heat-inactivated fetal bovine serum (Life Technologies, Carlsbad, California, USA) in minimal essential medium-α (Life Technologies) in a final count of 8.51 × 0 5 cells/ml and differentiated into osteoclasts using 10 nM dexamethasone and 10 nM vitamin D for 7 days. ..

    Article Title: IL-12/IL-23p40 identified as a downstream target of apremilast in ex vivo models of arthritis
    Article Snippet: Culture supernatants were analyzed for the concentration of IL-10 and MCP-1 by enzyme-linked immunosorbent assays (ELISAs; Biolegend, San Diego, California, USA) and for enzyme activity of tartrate-resistant acid phosphatase (TRAP) positive by an enzymatic assay (B-bridge International, Santa Clara, California, USA) following the instructions of the manufacturer. .. Human bone marrow mononuclear cells (Lonza, Walkersville, MA, USA) were cultured in 10% heat-inactivated fetal bovine serum (Life Technologies, Carlsbad, California, USA) in minimal essential medium-α (Life Technologies) in a final count of 8.51 × 05 cells/ml and differentiated into osteoclasts using 10 nM dexamethasone and 10 nM vitamin D for 7 days. ..

    Isolation:

    Article Title: Fast, multiplane line-scan confocal microscopy using axially distributed slits
    Article Snippet: Once beating was observed, cells were metabolically selected in no glucose, RPMI 1640 Medium supplemented with 4 mM DL-lactate (Sigma), then replated and maintained in RPMI 1640 Medium, GlutaMAX supplemented with B-27. .. Human mesenchymal stem cells (hMSCs) were isolated from Human Bone Marrow Mononuclear Cells (Lonza), and maintained in DMEM, low glucose medium (Gibco) with 10% Fetal Bovine Serum (Sigma) and 1% Penn-Strep. hMSCs were used between passages 4-7. ..

    Article Title: Voltage Imaging of Cardiac Cells and Tissue Using the Genetically Encoded Voltage Sensor Archon1
    Article Snippet: .. Personal Genome Project (PGP1) were isolated from male, 53 years of age, fibroblasts. hMSCs were isolated from purchased Human Bone Marrow Mononuclear Cells (Lonza), from a male, 39. .. iPSCs were screened for copy number variants and virtual karyotyping using Illumina HumanOmniExpress12v1 arrays. iPSCs were maintained in complete mTeSR1 medium (Stem Cell) and differentiated in monolayers into CMs using RPMI 1640 Medium, GlutaMAX (Gibco) supplemented with B-27 Supplement, minus insulin (Gibco).



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    ( A ) Single cell RNA <t>sequencing</t> analysis UMAP representation of plasma cells sorted from bone marrow of healthy donors. Shown are combined data from 3 donors. Heavy chain subclass information was extracted from VDJ sequencing annotations. Here, only IGHA1/2 are highlighted. Other IGHC isotypes can be seen in Supplementary Fig. 3. ( B ) Dot plot for expression of glycosyltransferases in IgHA1 and IgHA2 expressing plasma cells. Dot colors represent mean expression of the genes in each cell group and dot sizes indicate the percentage of cells expressing the respective genes. ( C ) Schematic overview of the contribution of selected enzymes to glycan processing. MAN1A2 is involved in trimming of the outer-arm mannose residues, B4GALT1 and ST6GAL1 add galactose and terminal sialic acid residues, respectively. ( D ) Flow cytometry analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the mean fluorescence intensity (MFI) for surface 2,6-sialic acid (measured by binding of sambuccus nigra agglutinine) and intracellular MAN1A2 and B4GALT1 (measured by binding of specific antibodies). Data are normalized on the MFI of IgA1 producing plasma cells. Every dot represents one donor. N=5-6. E) ELISpot analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the median and the 75 percentile of the spot size. Significances were tested with Wilcoxon rank test. * – p<0.05; ns – not significant. ( F ) Representative ELISpot images. Scale bar = 2 mm.
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    adult human bone marrow mononuclear cells ficoll-extracted  (Lonza)
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    ( A ) Single cell RNA <t>sequencing</t> analysis UMAP representation of plasma cells sorted from bone marrow of healthy donors. Shown are combined data from 3 donors. Heavy chain subclass information was extracted from VDJ sequencing annotations. Here, only IGHA1/2 are highlighted. Other IGHC isotypes can be seen in Supplementary Fig. 3. ( B ) Dot plot for expression of glycosyltransferases in IgHA1 and IgHA2 expressing plasma cells. Dot colors represent mean expression of the genes in each cell group and dot sizes indicate the percentage of cells expressing the respective genes. ( C ) Schematic overview of the contribution of selected enzymes to glycan processing. MAN1A2 is involved in trimming of the outer-arm mannose residues, B4GALT1 and ST6GAL1 add galactose and terminal sialic acid residues, respectively. ( D ) Flow cytometry analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the mean fluorescence intensity (MFI) for surface 2,6-sialic acid (measured by binding of sambuccus nigra agglutinine) and intracellular MAN1A2 and B4GALT1 (measured by binding of specific antibodies). Data are normalized on the MFI of IgA1 producing plasma cells. Every dot represents one donor. N=5-6. E) ELISpot analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the median and the 75 percentile of the spot size. Significances were tested with Wilcoxon rank test. * – p<0.05; ns – not significant. ( F ) Representative ELISpot images. Scale bar = 2 mm.
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    Image Search Results


    ( A ) Single cell RNA sequencing analysis UMAP representation of plasma cells sorted from bone marrow of healthy donors. Shown are combined data from 3 donors. Heavy chain subclass information was extracted from VDJ sequencing annotations. Here, only IGHA1/2 are highlighted. Other IGHC isotypes can be seen in Supplementary Fig. 3. ( B ) Dot plot for expression of glycosyltransferases in IgHA1 and IgHA2 expressing plasma cells. Dot colors represent mean expression of the genes in each cell group and dot sizes indicate the percentage of cells expressing the respective genes. ( C ) Schematic overview of the contribution of selected enzymes to glycan processing. MAN1A2 is involved in trimming of the outer-arm mannose residues, B4GALT1 and ST6GAL1 add galactose and terminal sialic acid residues, respectively. ( D ) Flow cytometry analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the mean fluorescence intensity (MFI) for surface 2,6-sialic acid (measured by binding of sambuccus nigra agglutinine) and intracellular MAN1A2 and B4GALT1 (measured by binding of specific antibodies). Data are normalized on the MFI of IgA1 producing plasma cells. Every dot represents one donor. N=5-6. E) ELISpot analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the median and the 75 percentile of the spot size. Significances were tested with Wilcoxon rank test. * – p<0.05; ns – not significant. ( F ) Representative ELISpot images. Scale bar = 2 mm.

    Journal: bioRxiv

    Article Title: IgA displays site- and subclass-specific glycoform differences despite equal glycoenzyme expression

    doi: 10.1101/2024.12.11.627887

    Figure Lengend Snippet: ( A ) Single cell RNA sequencing analysis UMAP representation of plasma cells sorted from bone marrow of healthy donors. Shown are combined data from 3 donors. Heavy chain subclass information was extracted from VDJ sequencing annotations. Here, only IGHA1/2 are highlighted. Other IGHC isotypes can be seen in Supplementary Fig. 3. ( B ) Dot plot for expression of glycosyltransferases in IgHA1 and IgHA2 expressing plasma cells. Dot colors represent mean expression of the genes in each cell group and dot sizes indicate the percentage of cells expressing the respective genes. ( C ) Schematic overview of the contribution of selected enzymes to glycan processing. MAN1A2 is involved in trimming of the outer-arm mannose residues, B4GALT1 and ST6GAL1 add galactose and terminal sialic acid residues, respectively. ( D ) Flow cytometry analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the mean fluorescence intensity (MFI) for surface 2,6-sialic acid (measured by binding of sambuccus nigra agglutinine) and intracellular MAN1A2 and B4GALT1 (measured by binding of specific antibodies). Data are normalized on the MFI of IgA1 producing plasma cells. Every dot represents one donor. N=5-6. E) ELISpot analysis of IgA1 and IgA2 producing bone marrow plasma cells. Shown is the median and the 75 percentile of the spot size. Significances were tested with Wilcoxon rank test. * – p<0.05; ns – not significant. ( F ) Representative ELISpot images. Scale bar = 2 mm.

    Article Snippet: For the single cell sequencing, cryopreserved human bone marrow mononuclear cells from three healthy donors were purchased from STEMCELL TM Technologies (5-50 Million cells per donor; viability > 93 %; two male African-Americans, 30 and 40 years old; one female Caucasian, 36 years old).

    Techniques: RNA Sequencing, Clinical Proteomics, Sequencing, Expressing, Glycoproteomics, Flow Cytometry, Fluorescence, Binding Assay, Enzyme-linked Immunospot